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Accuracy And Sensitivity Is Attainable Using Elisa Test Kits

By Sally Delacruz


Recently, scientists discovered a new method of diagnosing illnesses, antigens, antibodies and other foreign materials. Scientists normally mix reagents with samples of biological materials to be tested, and then wait for color change. Elisa test kits carry the tools used in carrying out tests for the existence of antigens and antibodies.

These kits are always made with the purpose of providing accuracy and consistency in research results. They are therefore required to be sensitive, easy to follow, precise and specific to individual tests. This can be easily achieved through rigorous testing. The testing may involve validating with many samples from, serum, saliva, feces and urine.

Accuracy is very important in making tests using these devices. They are, hence, calibrated to promote good vision which then translates into accuracy. Once calibration is properly done, the device will acquire sensitivity to the reagents used. Other factors observed in deciding on the best apparatus to use include; dynamism, ability to measure a wide range of readings, precision, and the ability to recover earlier readings.

These kits help in making research faster. A device that is deficient of the ability to reproduce results and sensitivity in making readings during research is not the best for Elisa test. Researchers might end up using a lot of money and time as a result of using these apparatus. This means that one needs to take a lot of caution while buying them from any company.

Besides antibodies, many other factors are crucial during the manufacture of these tools. For instance, rigorous antibody screening must be done, good standards chosen, steps for sample preparation developed and essays properly validated with the help of matrices. All these process are done with the purpose of attaining effectiveness.

The kits are capable of detecting items that are present in low concentrations for the sake of diagnosing and treating a number of diseases during their early stages. It has, previously been used in diagnosing different types of illnesses in both animals and plants. This technology has also been used for ensuring quality control in many industries.

The testing process involves attaching samples onto surfaces and later binding the antigens in samples with antibodies. Specific food substrates are then added. Color change seen in the ensuing reaction is the indicator of the absence or presence antigens.

When the kits are used in carrying out the same test, the tools on the kit are dipped into a test tube of samples collected. The set-up is then washed and then incubated in chromogens. The kit itself is made up of rods with about twelve ogives that protrude. The process of washing and incubating normally occurs when the ogive is immersed in small wells made of micro-plates containing reagents.

This kit is compatible with different types of reagents. Because of this quality, simultaneous tests for different antigens can be carried out. For this to be possible, the Ogive is sensitized to react to different reagents. The sensitivity of the tools can also be improved through raising the amount of samples.

The fact that these facilities can be used in establishing the amount of antibodies in the human body has made it possible for the test to be used in HIV testing. Their portability is also a cause for their popularity in carrying out such tests. Nevertheless, caution should be taken in determining the best kits.




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